We make use of PlasmidFactory‘s proprietary ITRRESCUE™ technology to amplify plasmid DNA containing sensitives ITR sequences
Please note in advance: The minimum order quantity for each plasmid is 5 mg.
General Background
Adeno-associated viruses (AAV) are currently gaining importance as vectors in gene therapy. AAV will cause no viral disease symptoms in the target organism, and only a mild immune response is to be expected. Integration into the host genome as a possible complication is also extremely rare.
Serotypes
Various so-called serotypes, created by modification of the binding motif on the viral capsids, allow for targeted and efficient transfection of cells or tissues and additionally increase, due to reduction of the potential for recognition by human serum antibodies, infection efficiency.
Application
The pDG plasmids for the production of adeno-associated virus (AAV) form a 2-plasmid system. This means that, in contrast to triple transfection with three different plasmids, only two components are needed: A plasmid with the gene to be transferred; and the corresponding pDG plasmid containing all the information for the propagation and packaging of AAV vector plasmids (the so-called «helper & packaging» functions). Additional infection with helper viruses – required in the original systems – is not necessary.
DKFZ-License
PlasmidFactory has obtained an exclusive, worldwide license for the production, distribution and use of the helper & packaging plasmids of the pDG and pDP families from the German Cancer Research Centre (DKFZ).
